Lee FK, Pereira L, Griffin C, Reid E, Nahmias A.Detection of herpes simplex virus type 2-specific antibody with glycoprotein G. Lee FK, Coleman RM, Pereira L, Bailey PD, Tatsuno M, Nahmias AJ.A trial of topical acyclovir in genital herpes simplex virus infections. Corey L, Nahmias AJ, Guinan ME, Benedetti JK, Critchlow CW, Holmes KK.Presence of type 2 antibody in acute serum samples. Serologic analysis of first-episode nonprimary genital herpes simplex virus infection. Humoral immune response to HSV-1 and HSV-2 viral proteins in patients with primary genital herpes. Use of densitometric analysis for interpreting HSV serologies based on Western blot. Detection of type-specific antibody to herpes simplex virus type 1 by radioimmunoassay with herpes simplex virus type 1 glycoprotein C purified with monoclonal antibody. Arvin AM, Koropchak CM, Yeager AS, Pereira L.Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Full textįull text is available as a scanned copy of the original print version. WBA was more sensitive than IEA in detecting seroconversion following primary HSV-1 genital herpes, although both assays may miss persons undergoing early seroconversion to HSV-2. Both WBA and IEA are accurate and sensitive tests for HSV-2 antibody in patients convalescing from a first episode or having symptomatic or asymptomatic recurrent genital herpes. In patients with culture-proven primary genital HSV-1 infection, WBA detected antibodies to HSV-1 proteins in 16 of 17 (94%) serum samples drawn at least 21 days after onset of primary genital HSV-1 infection, compared with 9 of 17 (53%) serum samples tested for gG-1 by IEA. Of sera drawn earlier than 21 days from onset of primary genital HSV-2, antibodies to HSV-2 were detected in 25% by WBA and 8% by IEA. Both tests were most sensitive with sera obtained at least 21 days after onset of primary HSV-2 infections or sera drawn during recurrent HSV-2 genital episodes: 75 of 76 (99%) such serum samples were positive for HSV-2 antibody by WBA and 73 of 76 (96%) were positive by IEA. Of 137 serum samples tested, none was mistyped by either WBA or IEA. Sera from patients with culture-proven genital herpes infections were tested for herpes simplex virus type 1 (HSV-1)- and HSV-2-specific antibodies by both a Western blot (immunoblot) technique (WBA) and immunodot enzyme assays (IEAs) specific for HSV-1 or HSV-2 glycoprotein G (gG).
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